Antimycobacterial activity of medicinal plants used by the Mayo people of Sonora, Mexico.

Autor: Coronado-Aceves EW; Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, 85000 Ciudad Obregón, Sonora, Mexico., Sánchez-Escalante JJ; Herbario USON, Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Niños Héroes entre Rosales y José María Pino Suárez, Edificio 1A (Museo), Colonia Centro, 83000 Hermosillo, Sonora, Mexico., López-Cervantes J; Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, 85000 Ciudad Obregón, Sonora, Mexico., Robles-Zepeda RE; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Encinas y Rosales, Colonia Centro, 83000 Hermosillo, Sonora, Mexico., Velázquez C; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Encinas y Rosales, Colonia Centro, 83000 Hermosillo, Sonora, Mexico., Sánchez-Machado DI; Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, 85000 Ciudad Obregón, Sonora, Mexico., Garibay-Escobar A; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Encinas y Rosales, Colonia Centro, 83000 Hermosillo, Sonora, Mexico. Electronic address: agaribay@guayacan.uson.mx.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2016 Aug 22; Vol. 190, pp. 106-15. Date of Electronic Publication: 2016 Jun 01.
DOI: 10.1016/j.jep.2016.05.064
Abstrakt: Ethnopharmacological Relevance: Tuberculosis (TB) is an infectious disease mainly caused by Mycobacterium tuberculosis (Mtb), which generates 9 million new cases worldwide each year. The Mayo ethnicity of southern Sonora, Mexico is more than 2000 years old, and the Mayos possess extensive knowledge of traditional medicine.
Aims of the Study: To evaluate the antimycobacterial activity levels of extracts of medicinal plants used by the Mayos against Mtb and Mycobacterium smegmatis (Msm) in the treatment of TB, respiratory diseases and related symptoms.
Materials and Methods: A total of 34 plant species were collected, and 191 extracts were created with n-hexane, dichloromethane, ethyl acetate (EtOAc), methanol and water. Their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined against Mtb H37Rv using the microplate alamar blue assay (MABA) and against Msm using the resazurin microplate assay (REMA) at 6 and 2 days of exposure, respectively, and at concentrations of 250-1.9µg/mL (n-hexane extracts) and 1000-7.81µg/mL (extracts obtained with dichloromethane, EtOAc, methanol and water).
Results: Rhynchosia precatoria (Willd.) DC. (n-hexane root extract), Euphorbia albomarginata Torr. and A. Gray. (EtOAc shoot extract) and Helianthus annuus L. (n-hexane stem extract) were the most active plants against Mtb H37Rv, with MICs of 15.6, 250, 250µg/mL and MBCs of 31.25, 250, 250µg/mL, respectively. R. precatoria (root) was the only active plant against Msm, with MIC and MBC values of ≥250µg/mL. None of the aqueous extracts were active.
Conclusions: This study validates the medicinal use of certain plants used by the Mayo people in the treatment of TB and related symptoms. R. precatoria, E. albomarginata and H. annuus are promising plant sources of active compounds that act against Mtb H37Rv. To our knowledge, this is the first time that their antimycobacterial activity has been reported. Crude extracts obtained with n-hexane, EtOAc and dichloromethane were the most active against Mtb H37Rv.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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