Quantitative IR microscopy and spectromics open the way to 3D digital pathology.

Autor: Bobroff V; University of Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint Hillaire, Bat B2, F33600, Pessac, France., Chen HH; University of Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint Hillaire, Bat B2, F33600, Pessac, France., Delugin M; University of Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint Hillaire, Bat B2, F33600, Pessac, France., Javerzat S; University of Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint Hillaire, Bat B2, F33600, Pessac, France., Petibois C; University of Bordeaux, Inserm U1029 LAMC, Allée Geoffroy Saint Hillaire, Bat B2, F33600, Pessac, France.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2017 Apr; Vol. 10 (4), pp. 598-606. Date of Electronic Publication: 2016 Jun 01.
DOI: 10.1002/jbio.201600051
Abstrakt: Currently, only mass-spectrometry (MS) microscopy brings a quantitative analysis of chemical contents of tissue samples in 3D. Here, the reconstruction of a 3D quantitative chemical images of a biological tissue by FTIR spectro-microscopy is reported. An automated curve-fitting method is developed to extract all intense absorption bands constituting IR spectra. This innovation benefits from three critical features: (1) the correction of raw IR spectra to make them quantitatively comparable; (2) the automated and iterative data treatment allowing to transfer the IR-absorption spectrum into a IR-band spectrum; (3) the reconstruction of an 3D IR-band matrix (x, y, z for voxel position and a 4 th dimension with all IR-band parameters). Spectromics, which is a new method for exploiting spectral data for tissue metadata reconstruction, is proposed to further translate the related chemical information in 3D, as biochemical and anatomical tissue parameters. An example is given with oxidative stress distribution and the reconstruction of blood vessels in tissues. The requirements of IR microscopy instrumentation to propose 3D digital histology as a clinical routine technology is briefly discussed.
(© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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