A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics.

Autor: Del Ben F; Graduate School of Nanotechnology, University of Trieste, Via Valerio 2, Trieste, Italy. fabio.delben@phd.units.it.; Dept. of Clinical Pathology, CRO Aviano, Via F. Gallini 2, 33081, Aviano, PN, Italy. fabio.delben@phd.units.it., Turetta M; Institute of Anatomic Pathology, Dept. of Medical and Biological Sciences, Azienda Ospedaliero-Universitaria di Udine, 33100, Udine, Italy., Celetti G; Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525AJ, Nijmegen, The Netherlands., Piruska A; Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525AJ, Nijmegen, The Netherlands., Bulfoni M; Institute of Anatomic Pathology, Dept. of Medical and Biological Sciences, Azienda Ospedaliero-Universitaria di Udine, 33100, Udine, Italy., Cesselli D; Institute of Anatomic Pathology, Dept. of Medical and Biological Sciences, Azienda Ospedaliero-Universitaria di Udine, 33100, Udine, Italy., Huck WT; Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525AJ, Nijmegen, The Netherlands. w.huck@science.ru.nl., Scoles G; Institute of Anatomic Pathology, Dept. of Medical and Biological Sciences, Azienda Ospedaliero-Universitaria di Udine, 33100, Udine, Italy. gscoles@princeton.edu.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Jul 18; Vol. 55 (30), pp. 8581-4. Date of Electronic Publication: 2016 Jun 01.
DOI: 10.1002/anie.201602328
Abstrakt: The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label-free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single-cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200 000 white blood cells and proof-of-concept data is shown on the detection of CTCs in the blood of metastatic patients.
(© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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