Selection of oleaginous yeasts for fatty acid production.
| Autor: | Lamers D; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands. dennis.lamers@han.nl.; Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands. dennis.lamers@han.nl., van Biezen N; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands., Martens D; Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands., Peters L; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands., van de Zilver E; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands., Jacobs-van Dreumel N; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands., Wijffels RH; Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands.; University of Nordland, Faculty of Biosciences and Aquaculture, N-8049, Bodø, Norway., Lokman C; HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands. |
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| Jazyk: | angličtina |
| Zdroj: | BMC biotechnology [BMC Biotechnol] 2016 May 27; Vol. 16 (1), pp. 45. Date of Electronic Publication: 2016 May 27. |
| DOI: | 10.1186/s12896-016-0276-7 |
| Abstrakt: | Background: Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels, which are based on non-food competing "waste carbon sources". Results: In this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were: a broad maximum temperature and pH range for growth (robustness of the strain), a broad spectrum of carbon sources that can be metabolized (preferably including C-5 sugars), a high total fatty acid content in combination with a low glycogen content and genetic accessibility. Conclusions: Based on these selection criteria, among 24 screened species, Schwanniomyces occidentalis (Debaromyces occidentalis) CBS2864 was selected as a promising strain for the production of high amounts of lipids. |
| Databáze: | MEDLINE |
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