Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications.

Autor:	Kashyap RS; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India. raj_ciims@rediffmail.com., Bhullar SS; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India., Chandak NH; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India., Taori GM; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1426, pp. 39-50.
DOI:	10.1007/978-1-4939-3618-2_4
Abstrakt:	Synthetic peptide-based diagnosis of Chikungunya can be an efficient and more accessible approach in immunodiagnostics. Here, we describe the identification of Chikungunya-specific 40 kD protein for development of synthetic peptide-based enzyme-linked immunosorbent assay for the detection of Chikungunya virus-specific antibodies in the patient's sample. The total sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profile of the patient's sample can be done to identify specific protein bands. The identified proteins can be subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for characterization. After characterization, immunogenic peptides can be designed using softwares and subsequently synthesized chemically. The peptides can be used to develop more specific, sensitive, and simpler diagnostic assay.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu