Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation.

Autor:	Baker CD; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843., Basu Ball W; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843., Pryce EN; Integrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, MD 21218., Gohil VM; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843 vgohil@tamu.edu.
Jazyk:	angličtina
Zdroj:	Molecular biology of the cell [Mol Biol Cell] 2016 Jul 15; Vol. 27 (14), pp. 2161-71. Date of Electronic Publication: 2016 May 25.
DOI:	10.1091/mbc.E15-12-0865
Abstrakt:	Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non-bilayer-forming phospholipid of mitochondria, results in disruption of MRC supercomplexes. However, the functions of the most abundant mitochondrial phospholipids, bilayer-forming phosphatidylcholine (PC) and non-bilayer-forming phosphatidylethanolamine (PE), are not clearly defined. Using yeast mutants of PE and PC biosynthetic pathways, we show a specific requirement for mitochondrial PE in MRC complex III and IV activities but not for their formation, whereas loss of PC does not affect MRC function or formation. Unlike CL, mitochondrial PE or PC is not required for MRC supercomplex formation, emphasizing the specific requirement of CL in supercomplex assembly. Of interest, PE biosynthesized in the endoplasmic reticulum (ER) can functionally substitute for the lack of mitochondrial PE biosynthesis, suggesting the existence of PE transport pathway from ER to mitochondria. To understand the mechanism of PE transport, we disrupted ER-mitochondrial contact sites formed by the ERMES complex and found that, although not essential for PE transport, ERMES facilitates the efficient rescue of mitochondrial PE deficiency. Our work highlights specific roles of non-bilayer-forming phospholipids in MRC function and formation. (© 2016 Baker et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)
Databáze:	MEDLINE
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