Eggs as a Suitable Tool for Species Diagnosis of Causative Agents of Human Diphyllobothriosis (Cestoda).

Autor: Leštinová K; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences & Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic., Soldánová M; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences & Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic., Scholz T; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences & Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic., Kuchta R; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences & Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2016 May 25; Vol. 10 (5), pp. e0004721. Date of Electronic Publication: 2016 May 25 (Print Publication: 2016).
DOI: 10.1371/journal.pntd.0004721
Abstrakt: Background: Tapeworms of the order Diphyllobothriidea are parasites of tetrapods and several species may infect man and cause neglected human disease called diphyllobothriosis. Identification of human-infecting diphyllobothriid cestodes is difficult because of their morphological uniformity, which concerns also their eggs in stool samples.
Methods: In the present study, we analysed by far the largest dataset of more than 2,000 eggs of 8 species of diphyllobothriid cestodes that may infect humans, including the most frequent human parasites Diphyllobothrium latum, D. nihonkaiense and Adenocephalus pacificus (syn. Diphyllobothrium pacificum). Size (length, width and length/width ratio) and the surface of the egg shell from naturally and experimentally infected hosts were studied using light and scanning electron microscopy.
Results: A high degree of intraspecific and host-related size variability has been detected, but combination of morphometrical and ultrastructural data made it possible to distinguish all of the studied species, including otherwise quite similar eggs of the 3 most common species infecting man, i.e. D. latum, D. nihonkaiense and D. dendriticum. The surface of all marine species is covered by numerous deep pits with species-specific density, whereas the surface of freshwater species is smooth or with isolated shallow hollows or wrinkles.
Databáze: MEDLINE
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