Dual behavior of N-acetylcysteine during ethanol-induced oxidative stress in embryonic chick brains.

Autor: Bauer AK; a Lake Erie College of Osteopathic Medicine , Erie , PA 16509 , USA., Fitzgerald M; b Michigan State University College of Human Medicine , East Lansing , USA., Ladzinski AT; c Midwestern University College of Osteopathic Medicine , Downers Grove , IL 60515 , USA., Lenhart Sherman S; d School of Dentistry , University of Michigan , Ann Arbor , USA., Maddock BH; e College of Medicine , University of Rochester , NY , USA., Norr ZM; f Biology Department , Hillsdale College , MI 49242 , USA., Miller RR Jr; f Biology Department , Hillsdale College , MI 49242 , USA.
Jazyk: angličtina
Zdroj: Nutritional neuroscience [Nutr Neurosci] 2017 Oct; Vol. 20 (8), pp. 478-488. Date of Electronic Publication: 2016 May 24.
DOI: 10.1080/1028415X.2016.1185261
Abstrakt: Objectives: Ethanol (EtOH) causes oxidative stress in embryos. Because N-acetylcysteine (NAC) failures and successes in ameliorating EtOH-induced oxidative stress have been reported, the objective was to determine if exogenous NAC ameliorated EtOH-induced oxidative stress within embryonic chick brains.
Methods: Control eggs were injected with approximately 25 µl of water on day 0, 1, and 2 of development (E 0-2 ). Experimental eggs were injected with dosages of either 3.0 mmol EtOH/kg egg; 747 µmol NAC/kg egg; 3.0 mmol EtOH and 747 µmol NAC/kg egg; 1000 µmol NAC/kg egg; or 3.0 mmol EtOH and 1000 µmol NAC/kg during the first 3 days of development (E 0-2 ). At 11 days of development (E 11 ; late embryogenesis), brains were harvested and subsequently assayed for oxidative stress markers including the loss of long-chain membrane polyunsaturated fatty acids (PUFAs); the accumulation of lipid hydroperoxides (LPO); decreased glutathione (GSH) and glutathione/glutathione disulfide (GSSG) levels; and decreased glutathione peroxidase (GPx) activities.
Results: EtOH (3 mmol/kg egg), medium NAC (747 µmol/kg egg), and EtOH and medium NAC promoted oxidative stress. These treatments caused decreased brain membrane long-chain PUFAs; increased LPO levels; decreased GSH levels and GSH/GSSG levels; and decreased Se-dependent GPx activities. High NAC dosages (1000 µmol/kg egg) attenuated EtOH-induced oxidative stress within EtOH and high NAC-treated chick brains.
Discussion: Exogenous EtOH and/or medium NAC propagated oxidative stress. Meanwhile, high NAC ameliorated EtOH-induced oxidative stress.
Databáze: MEDLINE
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