| Autor: |
Podosokorskaya OA; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Merkel AY; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Gavrilov SN; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Fedoseev I; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Heerden EV; TIA-UFS SAENSE Platform, University of the Free State, Bloemfontein, South Africa., Cason ED; TIA-UFS SAENSE Platform, University of the Free State, Bloemfontein, South Africa., Novikov AA; Gubkin Russian State, University of Oil and Gas, Moscow, Russia., Kolganova TV; Bioengineering Center, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Korzhenkov AA; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Bonch-Osmolovskaya EA; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia., Kublanov IV; Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia. |
| Abstrakt: |
A novel aerotolerant anaerobic, moderately thermophilic, organotrophic bacterium, strain MBL-TLPT, was isolated from a sample of microbial mat, developed under the flow of subsurface water in TauTona gold mine, South Africa. Cells of the new isolate were flagellated, spore-forming rods, 0.25-0.5 µm in width and 3-15 µm in length. Strain MBL-TLPT grew in the temperature range from 25 to 58 °C, pH range from 5.6 to 8.8 and at NaCl concentration from 0 to 85 g l-1. The isolate was able to ferment yeast extract and mono-, oligo- and polysaccharides, including starch and xanthan gum. The G+C content of the DNA was 35 mol%. Phylogenetic analysis of 16S rRNA gene sequences of strain MBL-TLPT and relatives showed its affiliation to the genus Tepidibacillus. Tepidibacillus fermentans STGHT was its closest relative (97.1 % identity of 16S rRNA gene sequences). Based on phylogenetic analysis and the physiological properties of the novel isolate, we propose a novel species, Tepidibacillus infernus sp. nov., with MBL-TLPT(=DSM 28123T=VKM В-2949T) as the type strain. |
