| Autor: |
Bonakdar S; National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran., Mahmoudi M; Department of Nanotechnology & Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences , P.O. Box 14155-6451, Tehran, Iran.; Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School , Boston, Massachusetts 02115, United States., Montazeri L; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran, Iran., Taghipoor M; Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland., Bertsch A; Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland., Shokrgozar MA; National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran., Sharifi S; MIRA Institute for Biomedical Technology and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente , P.O. Box 217, 7500 AE Enschede, The Netherlands., Majidi M; National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran., Mashinchian O; Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland., Hamrang Sekachaei M; National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran., Zolfaghari P; National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran., Renaud P; Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland. |
| Abstrakt: |
Differentiation of stem cells into mature cells through the use of physical approaches is of great interest. Here, we prepared smart nanoenvironments by cell-imprinted substrates based on chondrocytes, tenocytes, and semifibroblasts as templates and demonstrated their potential for differentiation, redifferentiation, and transdifferentiation. Analysis of shape and upregulation/downregulation of specific genes of stem cells, which were seeded on these cell-imprinted substrates, confirmed that imprinted substrates have the capability to induce specific shapes and molecular characteristics of the cell types that were used as templates for cell-imprinting. Interestingly, immunofluorescent staining of a specific protein in chondrocytes (i.e., collagen type II) confirmed that adipose-derived stem cells, semifibroblasts, and tenocytes can acquire the chondrocyte phenotype after a 14 day culture on chondrocyte-imprinted substrates. In summary, we propose that common polystyrene tissue culture plates can be replaced by this imprinting technique as an effective and promising way to regulate any cell phenotype in vitro with significant potential applications in regenerative medicine and cell-based therapies. |
