Irc3 is a mitochondrial DNA branch migration enzyme.
| Autor: | Gaidutšik I; Institute of Molecular and Cell Biology, University of Tartu, Riia 23b, Tartu 51010, Estonia., Sedman T; Institute of Molecular and Cell Biology, University of Tartu, Riia 23b, Tartu 51010, Estonia., Sillamaa S; Institute of Molecular and Cell Biology, University of Tartu, Riia 23b, Tartu 51010, Estonia., Sedman J; Institute of Molecular and Cell Biology, University of Tartu, Riia 23b, Tartu 51010, Estonia. |
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| Jazyk: | angličtina |
| Zdroj: | Scientific reports [Sci Rep] 2016 May 19; Vol. 6, pp. 26414. Date of Electronic Publication: 2016 May 19. |
| DOI: | 10.1038/srep26414 |
| Abstrakt: | Integrity of mitochondrial DNA (mtDNA) is essential for cellular energy metabolism. In the budding yeast Saccharomyces cerevisiae, a large number of nuclear genes influence the stability of mitochondrial genome; however, most corresponding gene products act indirectly and the actual molecular mechanisms of mtDNA inheritance remain poorly characterized. Recently, we found that a Superfamily II helicase Irc3 is required for the maintenance of mitochondrial genome integrity. Here we show that Irc3 is a mitochondrial DNA branch migration enzyme. Irc3 modulates mtDNA metabolic intermediates by preferential binding and unwinding Holliday junctions and replication fork structures. Furthermore, we demonstrate that the loss of Irc3 can be complemented with mitochondrially targeted RecG of Escherichia coli. We suggest that Irc3 could support the stability of mtDNA by stimulating fork regression and branch migration or by inhibiting the formation of irregular branched molecules. |
| Databáze: | MEDLINE |
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