The Type I Restriction Enzymes as Barriers to Horizontal Gene Transfer: Determination of the DNA Target Sequences Recognised by Livestock-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complexes 133/ST771 and 398.

Autor:	Chen K; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK.; Shenyang Research Institute of Chemical Industry, 8 Shenliao Dong Road, Shenyang, Liaoning, People's Republic of China., Stephanou AS; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK., Roberts GA; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK., White JH; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK., Cooper LP; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK., Houston PJ; Institute of Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE, UK.; The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 0NF, UK., Lindsay JA; Institute of Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE, UK. jlindsay@sgul.ac.uk., Dryden DT; EaStCHEM School of Chemistry, University of Edinburgh the King's Buildings, Edinburgh, EH9 3JJ, UK. david.dryden@ed.ac.uk.
Jazyk:	angličtina
Zdroj:	Advances in experimental medicine and biology [Adv Exp Med Biol] 2016; Vol. 915, pp. 81-97.
DOI:	10.1007/978-3-319-32189-9_7
Abstrakt:	The Type I DNA restriction-modification (RM) systems of Staphylococcus aureus are known to act as a significant barrier to horizontal gene transfer between S. aureus strains belonging to different clonal complexes. The livestock-associated clonal complexes CC133/771 and CC398 contain Type I RM systems not found in human MRSA strains as yet but at some point transfer will occur. When this does take place, horizontal gene transfer of resistance will happen more easily between these strains. The reservoir of antibiotic resistance, virulence and host-adaptation genes present in livestock-associated MRSA will then potentially contribute to the development of newly evolving MRSA clones. The target sites recognised by the Type I RM systems of CC133/771 and CC398 were identified as CAG(N)5RTGA and ACC(N)5RTGA, respectively. Assuming that these enzymes recognise the methylation state of adenine, the underlined A and T bases indicate the unique positions of methylation. Target methylation points for enzymes from CC1 were also identified. The methylation points for CC1-1 are CCAY(N)5TTAA and those for CC1-2 are CCAY(N)6 TGT with the underline indicating the adenine methylation site thus clearing up the ambiguity noted previously (Roberts et al. 2013, Nucleic Acids Res 41:7472-7484) for the half sites containing two adenine bases.
Databáze:	MEDLINE
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