Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.

Autor: Kilianski A, Roth PA, Liem AT, Hill JM, Willis KL, Rossmaier RD, Marinich AV, Maughan MN, Karavis MA, Kuhn JH, Honko AN, Rosenzweig CN
Jazyk: angličtina
Zdroj: Emerging infectious diseases [Emerg Infect Dis] 2016 Aug; Vol. 22 (8), pp. 1448-51. Date of Electronic Publication: 2016 Aug 15.
DOI: 10.3201/eid2208.160270
Abstrakt: Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.
Databáze: MEDLINE