The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells.

Autor:	Chang GW; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 333 Tao-Yuan, Taiwan., Hsiao CC; Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands., Peng YM; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 333 Tao-Yuan, Taiwan., Vieira Braga FA; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, the Netherlands., Kragten NA; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, the Netherlands., Remmerswaal EB; Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands; Renal Transplant Unit, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands., van de Garde MD; Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands., Straussberg R; Department of Child Neurology, Neurogenetics Clinic, Schneider Children's Medical Center, Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel., König GM; Institute for Pharmaceutical Biology, University of Bonn, 53115 Bonn, Germany., Kostenis E; Institute for Pharmaceutical Biology, University of Bonn, 53115 Bonn, Germany., Knäuper V; Dental School, Cardiff University, Cardiff CF14 4XN, UK., Meyaard L; Department of Immunology, University Medical Center Utrecht, 3584 EA Utrecht, the Netherlands., van Lier RA; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, the Netherlands., van Gisbergen KP; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, the Netherlands., Lin HH; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 333 Tao-Yuan, Taiwan; Chang Gung Immunology Consortium and Department of Anatomic Pathology, Chang Gung Memorial Hospital-Linkou, 333 Tao-Yuan, Taiwan. Electronic address: hhlin@mail.cgu.edu.tw., Hamann J; Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands. Electronic address: j.hamann@amc.uva.nl.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2016 May 24; Vol. 15 (8), pp. 1757-70. Date of Electronic Publication: 2016 May 12.
DOI:	10.1016/j.celrep.2016.04.053
Abstrakt:	Natural killer (NK) cells possess potent cytotoxic mechanisms that need to be tightly controlled. Here, we explored the regulation and function of GPR56/ADGRG1, an adhesion G protein-coupled receptor implicated in developmental processes and expressed distinctively in mature NK cells. Expression of GPR56 was triggered by Hobit (a homolog of Blimp-1 in T cells) and declined upon cell activation. Through studying NK cells from polymicrogyria patients with disease-causing mutations in ADGRG1, encoding GPR56, and NK-92 cells ectopically expressing the receptor, we found that GPR56 negatively regulates immediate effector functions, including production of inflammatory cytokines and cytolytic proteins, degranulation, and target cell killing. GPR56 pursues this activity by associating with the tetraspanin CD81. We conclude that GPR56 inhibits natural cytotoxicity of human NK cells. (Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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