The endochitinase ChiA Btt of Bacillus thuringiensis subsp. tenebrionis DSM-2803 and its potential use to control the phytopathogen Colletotrichum gloeosporioides.

Autor: de la Fuente-Salcido NM; Aniversidad Autónoma de Coahuila, Escuela de Ciencias Biológicas, Torreón, Coahuila, 27104, México.; Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México., Casados-Vázquez LE; Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.; Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México., García-Pérez AP; Aniversidad Autónoma de Coahuila, Escuela de Ciencias Biológicas, Torreón, Coahuila, 27104, México., Barboza-Pérez UE; Tecnológico de Monterrey Campus Querétaro, Epigmenio González 500 Fracc, San Pablo, Querétaro, Qro, 76130, México., Bideshi DK; Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, 92504, California.; Department of Entomology, University of California, Riverside, California, 92521., Salcedo-Hernández R; Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.; Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México., García-Almendarez BE; Universidad Autónoma de Querétaro. DIPA, PROPAC, Facultad de Química, Querétaro, Qro, 76010, México., Barboza-Corona JE; Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México. josebar@ugto.mx.; Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México. josebar@ugto.mx.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2016 Oct; Vol. 5 (5), pp. 819-829. Date of Electronic Publication: 2016 May 12.
DOI: 10.1002/mbo3.372
Abstrakt: Bacillus thuringiensis subsp. tenebrionis DSM-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. The endochitinase chiA Btt gene of B. thuringiensis subsp. tenebrionis DSM-2803 was cloned and expressed in Escherichia coli. The recombinant 6x-histidine tagged protein (rChiA Btt, ~74 kDa), was purified by a HiTrap Ni affinity column. The K m of rChiA Btt was 0.847 μmol L -1 and its optimal activity occurred at pH 7 and ~40°C. Most divalent cations reduced endochitinase activity but only Hg +2 abolished activity of the enzyme. We report for the first time the characterization of a chitinase synthesized by B. thuringiensis subsp. tenebrionis DSM-2803, and show that the purified rChiA74 Btt reduced the radial growth and increased the hyphal density of Colletotrichium gloeosporioides, the etiological agent of "anthracnose" in plants.
(© 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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