Formation of multimers of bacterial collagens through introduction of specific sites for oxidative crosslinking.

Autor: Stoichevska V; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia., An B; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155., Peng YY; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia., Yigit S; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155., Vashi AV; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia., Kaplan DL; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155., Werkmeister JA; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia., Dumsday GJ; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia., Ramshaw JA; CSIRO Manufacturing, Bayview Avenue, Clayton, VIC, 3169, Australia.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2016 Sep; Vol. 104 (9), pp. 2369-76. Date of Electronic Publication: 2016 May 26.
DOI: 10.1002/jbm.a.35772
Abstrakt: A range of non-animal collagens has been described, derived from bacterial species, which form stable triple-helical structures without the need for secondary modification to include hydroxyproline in the sequence. The non-animal collagens studied to date are typically smaller than animal interstitial collagens, around one quarter the length and do not pack into large fibrillar aggregates like those that are formed by the major animal interstitial collagens. A consequence of this for biomedical products is that fabricated items, such as collagen sponges, are not as mechanically and dimensionally stable as those of animal collagens. In the present study, we examined the production of larger, polymeric forms of non-animal collagens through introduction of tyrosine and cysteine residues that can form selective crosslinks through oxidation. These modifications allow the formation of larger aggregates of the non-animal collagens. When Tyr residues were incorporated, gels were obtained. And with Cys soluble aggregates were formed. These materials can be formed into sponges that are more stable than those formed without these modifications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2369-2376, 2016.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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