Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12.

Autor:	Portal TM; Programa de Pós-Graduação em Biologia Parasitária na Amazônia, Universidade do Estado do Pará, Belém, Pará, Brazil., Siqueira JA; Programa de Pós-Graduação em Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Costa LC; Faculdade Integrada Brasil Amazônia, Belém, Pará, Brazil., Lima IC; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Lucena MS; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Bandeira Rda S; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Linhares Ada C; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Luz CR; Universidade Federal do Maranhão, São Luís, Maranhão, Brazil., Gabbay YB; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Resque HR; Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil. Electronic address: hugoresque@iec.pa.gov.br.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2016 Jul-Sep; Vol. 47 (3), pp. 724-30. Date of Electronic Publication: 2016 Apr 22.
DOI:	10.1016/j.bjm.2016.04.008
Abstrakt:	Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p<0.01). Of the diarrheic patients, 17% had fever, vomiting and anorexia, and 13% developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50% (12/24) were sequenced and classified as genotypes GII.3 (n=1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8% (11/112), with 22.6% (7/31) in diarrheic patients and 4.9% (4/81) in nondiarrheic (p=0.04) ones. In diarrheic cases, 27.3% had fever, vomiting and anorexia, whereas 18.2% had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão. (Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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