[Effects of fine particulate matter from on bronchial epithelial cells].

Autor:	Aloui R; Service de pneumologie, centre hospitalier Lyon Sud, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite cedex, France., Magne F; Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, hôpital de la Croix-Rousse, 69000 Lyon, France., Devouassoux G; Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, hôpital de la Croix-Rousse, 69000 Lyon, France., Deverchere J; Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France., Ritter P; Direction de l'écologie urbaine, mairie de Lyon, 69205 Lyon cedex 01, France., Bentaher A; Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France., Pacheco Y; Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, centre hospitalier Lyon Sud, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite cedex, France. Electronic address: yves.pacheco@univ-lyon1.fr.
Jazyk:	francouzština
Zdroj:	Revue des maladies respiratoires [Rev Mal Respir] 2016 Nov; Vol. 33 (9), pp. 767-774. Date of Electronic Publication: 2016 May 04.
DOI:	10.1016/j.rmr.2016.02.010
Abstrakt:	Introduction: Ambient air contains a variety of pollutants including solid particles. Human bronchial epithelial cells (HBEC) play a key role following inhalation of these toxic particles. In a cell culture system of HBEC, we investigated the effects of several fractions of Lyons air-derived particles on cell viability/proliferation and production of the inflammatory mediators IL-8 and TGF-β. Methods: Air particles were collected in Lyon (France) by filtration of the air and impaction on filters located on a tapered element oscillating microbalance. Several diameter-based fractions of particles were prepared. HBEC were then exposed to different concentrations of these fractions. Results: HBEC survival was not altered after 48hours of culture in the presence of particles regardless of their diameter and concentration. A dose-dependent inhibitory effect on cell growth was observed for all fractions. The particles caused an increase of both TGF-ß and Il-8 protein levels as a function of their diameters and/or concentrations. Conclusion: Air-derived particle fractions exhibit both an inhibitory effect on cell proliferation and a pro-inflammatory activity on HBEC. (Copyright © 2016 SPLF. Published by Elsevier Masson SAS. All rights reserved.)
Databáze:	MEDLINE
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