Chemically sulfated natural galactomannans with specific antiviral and anticoagulant activities.

Autor: Muschin T; Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho Kitami 090-8507, Hokkaido, Japan; College of Chemistry and Environmental Science, Inner Mongolia Key Laboratory of Green catalysis, Inner Mongolia Normal University, Hohhot 010022, China., Budragchaa D; Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho Kitami 090-8507, Hokkaido, Japan., Kanamoto T; St. Marianna University School of Medicine, Miyamae-ku, Kawasaki 216-8511, Japan., Nakashima H; St. Marianna University School of Medicine, Miyamae-ku, Kawasaki 216-8511, Japan., Ichiyama K; Translational ID Lab, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, #15-02 Centre for Translational Medicine (MD6), Singapore 117599, Singapore, Singapore., Yamamoto N; Translational ID Lab, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, #15-02 Centre for Translational Medicine (MD6), Singapore 117599, Singapore, Singapore., Shuqin H; Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho Kitami 090-8507, Hokkaido, Japan., Yoshida T; Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho Kitami 090-8507, Hokkaido, Japan. Electronic address: yoshida@chem.kitami-it.ac.jp.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2016 Aug; Vol. 89, pp. 415-20. Date of Electronic Publication: 2016 May 03.
DOI: 10.1016/j.ijbiomac.2016.05.005
Abstrakt: Naturally occurring galactomannans were sulfated to give sulfated galactomannans with degrees of substitution of 0.7-1.4 per sugar unit and molecular weights of M¯n=0.6×10(4)-2.4×10(4). Sulfated galactomannans were found to have specific biological activities in vitro such as anticoagulant, anti-HIV and anti-Dengue virus activities. The biological activities were compared with those of standard dextran and curdlan sulfates, which are polysaccharides with potent antiviral activity and low cytotoxicity. It was found that sulfated galactomannans had moderate to high anticoagulant activity, 13.4-36.6unit/mg, compared to that of dextran and curdlan sulfates, 22.7 and 10.0unit/mg, and high anti-HIV and anti-Dengue virus activities, 0.04-0.8μg/mL and 0.2-1.1μg/mL, compared to those curdlan sulfates, 0.1μg/mL, respectively. The cytotoxicity on MT-4 and LCC-MK2 cells was low. Surface plasmon resonance (SPR) of sulfated galactomannans revealed strong interaction with poly-l-lysine as a model compound of virus proteins, and suggested that the specific biological activities might originate in the electrostatic interaction of negatively charged sulfate groups of sulfated galactomannans and positively charged amino groups of surface proteins of viruses. These results suggest that sulfated galactomannans effectively prevented the infection of cells by viruses and the degree of substitution and molecular weights played important roles in the biological activities.
(Copyright © 2016. Published by Elsevier B.V.)
Databáze: MEDLINE
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