| Autor: |
Gerding TK; Department of Toxicology, State University, Groningen, The Netherlands., Drenth BF, Van de Grampel VJ, Niemeijer NR, De Zeeuw RA, Tepper PG, Horn AS |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1989 Jan 27; Vol. 487 (1), pp. 125-34. |
| DOI: |
10.1016/s0378-4347(00)83014-5 |
| Abstrakt: |
This paper describes an enzymic derivatization procedure that allows accurate determination of very small amounts of enantiomeric impurities in the D-2 dopamine agonist 2-(N-propyl-N-2-thienylethylamino)-5-hydroxytetralin (N-0437). After pre-column glucuronidation of the individual enantiomers, two diastereoisomers were formed which were separated by reversed-phase high-performance liquid chromatography. An enantiomeric purity of 99.84% was calculated for the (-)-enantiomer, against 99.89% for the (+)-enantiomer. The assay was validated by spiking 1% of the (-)-enantiomer in the (+)-enantiomer. A high accuracy (error 4.5%) and precision (coefficient of variation 2.9%, n = 5) of the method were established. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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