Initial peptidomic profiling of Brazilian sea urchins: Arbacia lixula, Lytechinus variegatus and Echinometra lucunter.

Autor: Sciani JM; Laboratory of Biochemistry and Biophysics, Butantan Institute, Av. Vital Brasil, 1500, São Paulo, SP CEP 05503-900 Brazil., Emerenciano AK; Laboratory of Evolutionary Histophysiology, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP Brazil., Cunha da Silva JR; Laboratory of Evolutionary Histophysiology, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP Brazil., Pimenta DC; Laboratory of Biochemistry and Biophysics, Butantan Institute, Av. Vital Brasil, 1500, São Paulo, SP CEP 05503-900 Brazil.
Jazyk: angličtina
Zdroj: The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2016 May 04; Vol. 22, pp. 17. Date of Electronic Publication: 2016 May 04 (Print Publication: 2016).
DOI: 10.1186/s40409-016-0071-x
Abstrakt: Background: Sea urchins can be found throughout the Brazilian coast and are reported to be one of the major causes of marine accidents on the shoreline. Although not lethal, these accidents are reported to be extremely painful. In order to understand the toxinology of the Brazilian urchins, a peptidomic approach was performed aiming to characterize the naturally occurring peptides in both the coelomic fluid and the spine.
Methods: Animals were collected without gender distinction and samples of the coelomic fluid and spines extracted were analyzed by RP-HPLC and mass spectrometry for peptide de novo sequencing.
Results: Several peptides were identified either in the coelomic fluid or the spine extract (except for E. lucunter). The peptide sequences were aligned with public deposited sequences and possible functions were inferred. Moreover, some peptides can be cryptides, since their sequences were identified within functional proteins, for example thymosin from Strongylocentrotus purpuratus.
Conclusions: Although preliminary, the peptidomic approach presented here reports, for the first time, the abundance of novel biological molecules derived from these animals. The discovery of such molecules may be of potential biotechnological application, as described for other organisms; nevertheless, further studies are required.
Databáze: MEDLINE
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