Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in São Paulo, Brazil.

Autor:	Betiol JC; Molecular Biology Laboratory, Center of Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil (ICESP), São Paulo, Brazil., Sichero L; Molecular Biology Laboratory, Center of Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil (ICESP), São Paulo, Brazil.; Center of Translational Oncology - ICESP, Av. Dr. Arnaldo, 251, 8 andar, 01246-000, Cerqueira César, São Paulo, SP Brazil., Costa HOO; Department of Otolaringology, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., de Matos LL; Department of Head and Neck Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil., Andreoli MA; HPV Institute, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Ferreira S; Molecular Biology Laboratory, Center of Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil (ICESP), São Paulo, Brazil., Faraj SF; Department of Pathology, Cancer Institute of São Paulo (ICESP), São Paulo, Brazil., de Mello ES; Department of Pathology, Cancer Institute of São Paulo (ICESP), São Paulo, Brazil., Sobrinho JS; Molecular Biology Laboratory, Center of Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil (ICESP), São Paulo, Brazil., Brandão LG; Department of Head and Neck Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil., Cernea CR; Department of Head and Neck Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil., Kulcsar MA; Department of Head and Neck Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil., Pinto FR; Department of Head and Neck Surgery, School of Medicine, University of São Paulo, São Paulo, Brazil., Gonçalves AJ; Department of Surgery, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Menezes MB; Department of Surgery, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Silva L; Department of Otolaringology, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Rossi LM; Department of Otolaringology, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Nunes RAL; HPV Institute, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Termini L; HPV Institute, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil., Villa LL; Molecular Biology Laboratory, Center of Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil (ICESP), São Paulo, Brazil.; HPV Institute, Santa Casa de Sao Paulo, School of Medicine (FCMSCSP), São Paulo, Brazil.; Department of Radiology and Oncology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Infectious agents and cancer [Infect Agent Cancer] 2016 May 04; Vol. 11, pp. 20. Date of Electronic Publication: 2016 May 04 (Print Publication: 2016).
DOI:	10.1186/s13027-016-0067-8
Abstrakt:	Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in São Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Plný text ve formátu PDF