Proteome Changes during Transition from Human Embryonic to Vascular Progenitor Cells.

Autor: Tsolis KC; Department of Protein structure and Proteomics Facility, Institute of Molecular Biology and Biotechnology - FORTH , 70013 Iraklio, Crete, Greece.; Department of Biology, University of Crete , 70013 Iraklio, Crete, Greece., Bagli E; Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - FORTH , 45110 Ioaninna, Greece., Kanaki K; Department of Protein structure and Proteomics Facility, Institute of Molecular Biology and Biotechnology - FORTH , 70013 Iraklio, Crete, Greece., Zografou S; Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - FORTH , 45110 Ioaninna, Greece., Carpentier S; SYBIOMA, KU Leuven facility for Systems Biology Based Mass Spectrometry , B-3000 Leuven Belgium., Bei ES; School of Electronic and Computer Engineering, Technical University of Crete , 73100 Chania, Greece., Christoforidis S; Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - FORTH , 45110 Ioaninna, Greece.; Laboratory of Biological Chemistry, Medical School, University of Ioannina , 45110 Ioannina, Greece., Zervakis M; School of Electronic and Computer Engineering, Technical University of Crete , 73100 Chania, Greece., Murphy C; Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - FORTH , 45110 Ioaninna, Greece.; School of Biosciences, College of Life and Environmental Sciences, University of Birmingham , Edgbaston, Birmingham B15 2TT, U.K., Fotsis T; Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - FORTH , 45110 Ioaninna, Greece.; Laboratory of Biological Chemistry, Medical School, University of Ioannina , 45110 Ioannina, Greece.; School of Biosciences, College of Life and Environmental Sciences, University of Birmingham , Edgbaston, Birmingham B15 2TT, U.K., Economou A; Department of Protein structure and Proteomics Facility, Institute of Molecular Biology and Biotechnology - FORTH , 70013 Iraklio, Crete, Greece.; Department of Biology, University of Crete , 70013 Iraklio, Crete, Greece.; SYBIOMA, KU Leuven facility for Systems Biology Based Mass Spectrometry , B-3000 Leuven Belgium.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2016 Jun 03; Vol. 15 (6), pp. 1995-2007. Date of Electronic Publication: 2016 May 13.
DOI: 10.1021/acs.jproteome.6b00180
Abstrakt: Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34(+) vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34(-) outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34(+) and CD34(-) populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34(+) and CD34(-) cells. CD34(+) cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34(-) cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34(+) or CD34(-) cells raise testable hypotheses for future studies to probe vascular morphogenesis.
Databáze: MEDLINE
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