Effect of benzophenone-1 and octylphenol on the regulation of epithelial-mesenchymal transition via an estrogen receptor-dependent pathway in estrogen receptor expressing ovarian cancer cells.

Autor: Shin S; Laboratory of Biochemistry and Immunology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea., Go RE; Laboratory of Biochemistry and Immunology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea., Kim CW; Laboratory of Biochemistry and Immunology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea., Hwang KA; Laboratory of Biochemistry and Immunology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea., Nam KH; Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea., Choi KC; Laboratory of Biochemistry and Immunology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea. Electronic address: kchoi@cbu.ac.kr.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2016 Jul; Vol. 93, pp. 58-65. Date of Electronic Publication: 2016 May 01.
DOI: 10.1016/j.fct.2016.04.026
Abstrakt: Epithelial-mesenchymal transition (EMT) is an important process in embryonic development and cancer progression and metastasis. EMT is influenced by 17β-estradiol (E2), an endogenous estrogen. Benzophenone-1 (2,4-dihydroxybenzophenone, BP-1) and 4-tert-octylphenol (OP) are suspected endocrine disrupting chemicals (EDCs) because they can exhibit estrogenic properties. In this study, we examined whether BP-1 and OP can lead to EMT of BG-1 ovarian cancer cells expressing estrogen receptors (ERs). A wound healing assay and western blot assay were conducted to show the effect of BP-1 and OP on the migration of BG-1 cells and protein expression of EMT-related genes. BP-1 (10(-6) M) and OP (10(-6) M) significantly enhanced the migration capability of BG-1 cells by reducing the wounded area in the cell monolayer relative to the control, similar to E2 (10(-9) M). However, when BG-1 cells were co-treated with ICI 182,780, an ER antagonist, the uncovered area was maintained at the level of the control. N-cadherin, snail, and slug were increased by BP-1 and OP while E-cadherin was reduced compared to the control. However, this effect was also restored by co-treatment with ICI 182,780. Taken together, these results indicate that BP-1 and OP, the potential EDCs, may have the ability to induce ovarian cancer metastasis via regulation of the expression of EMT markers and migration of ER-expressing BG-1 ovarian cancer cells.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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