Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).

Autor: Weremijewicz J; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA; Department of Biology, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33126 USA., Almonte JI; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Hilaire VS; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Lopez FD; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Lu SH; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Marrero SM; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Martinez CM; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Zarate EA; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Lam AK; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Ferguson SA; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Petrakis NZ; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Peeples KA; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Taylor ED; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Leon NM; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Valdes C; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Hass M; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Reeve AB; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Palow DT; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA., Downing JL; Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA; Department of Biological Sciences, Florida International University, 11200 SW 8th Street, Miami, Florida 33199 USA.
Jazyk: angličtina
Zdroj: Applications in plant sciences [Appl Plant Sci] 2016 Apr 11; Vol. 4 (4). Date of Electronic Publication: 2016 Apr 11 (Print Publication: 2016).
DOI: 10.3732/apps.1500095
Abstrakt: Premise of the Study: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami's urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).
Methods and Results: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (H o) of 0.314 and average expected heterozygosity (H e) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average H o of 0.35 and an average H e of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average H o of 0.257 and an average H e of 0.272.
Conclusions: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project.
Databáze: MEDLINE
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