In vivo diagnosis with purified tropomyosin in mite and shellfish allergic patients.

Autor:	López-Matas MA; R&D Department, Laboratorios LETI S.L., Tres Cantos, Madrid, Spain., de Larramendi CH; Allergy Section, Hospital Marina Baixa, Villajoyosa and Centro de Especialidades Foietes, Benidorm, Alicante, Spain., Moya R; R&D Department, Laboratorios LETI S.L., Tres Cantos, Madrid, Spain., Sánchez-Guerrero I; Allergy Service, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain., Ferrer A; Allergy Unit, Hospital General Universitario de Elche, Elche, Alicante, Spain., Huertas AJ; Allergy Section, Complejo Hospitalario Universitario de Cartagena, Cartagena, Murcia, Spain., Flores I; Allergy Unit, Hospital de la Vega Baja, Orihuela, Alicante, Spain., Navarro LA; Allergy Unit, Centro de Especialidades El Españoleto, Játiva, Valencia, Spain., García-Abujeta JL; Allergy Section, Hospital Marina Baixa, Villajoyosa and Centro de Especialidades Foietes, Benidorm, Alicante, Spain., Vicario S; Allergy Section, Hospital Marina Baixa, Villajoyosa and Centro de Especialidades Foietes, Benidorm, Alicante, Spain., Andreu C; Allergy Unit, Hospital de la Vega Baja, Orihuela, Alicante, Spain., Peña M; Allergy Unit, Hospital de la Vega Baja, Orihuela, Alicante, Spain., Carnés J; R&D Department, Laboratorios LETI S.L., Tres Cantos, Madrid, Spain. Electronic address: jcarnes@leti.com.
Jazyk:	angličtina
Zdroj:	Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology [Ann Allergy Asthma Immunol] 2016 Jun; Vol. 116 (6), pp. 538-43. Date of Electronic Publication: 2016 Apr 28.
DOI:	10.1016/j.anai.2016.03.034
Abstrakt:	Background: Tropomyosin is the most studied shellfish allergen and has been involved in cross-reactivity among different invertebrates (crustacean, mollusks, mites, insects, and nematodes). Objective: To determine the relevance of tropomyosin in mite- and shellfish-sensitized patients using tropomyosin skin testing. Methods: Patients were divided into 3 groups: group M included mite allergic patients (ie, individuals with respiratory symptoms and a positive result on skin prick testing [SPT] to house dust mites), group S included shellfish allergic patients (ie, individuals who reported symptoms with shellfish), and group MS included mite- and shellfish allergic patients (ie, individuals who simultaneously fulfilled the inclusion criteria for groups M and S). Tropomyosin was purified from shrimp, characterized, and used in SPT for diagnosis in the patient population. Results: Eight hundred fifty patients were included in the study: 790 (92.9%) in group M, 21 (2.5%) in group S, and 39 (4.6%) in group MS. Tropomyosin was purified from shrimp with a purity higher than 95%. Forty-two individuals tested positive to tropomyosin: the prevalence was 2.7% in group M, 28.6% in group S, and 38.5% in patients of group MS. Twenty-one (50%) of the tropomyosin-positive individuals had symptoms with shellfish, and 3 (14.3%) reported anaphylaxis. Conclusion: The prevalence of tropomyosin was low in mite-sensitized patients (2.7 %) and high in shellfish allergic patients (28.6%). The higher prevalence of tropomyosin was found in patients sensitized to both mite and shellfish (38.5%). The selection of tropomyosin-sensitized patients by SPT might help in the choice of appropriate treatments or management for these patients. (Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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