Spherical gold nanoparticles and gold nanorods for the determination of gentamicin.

Autor: Miranda-Andrades JR; Chemistry Department, Pontifícia Universidade Católica do Rio de Janeiro, 22451-900, Rio de Janeiro, RJ, Brazil., Pérez-Gramatges A; Chemistry Department, Pontifícia Universidade Católica do Rio de Janeiro, 22451-900, Rio de Janeiro, RJ, Brazil., Pandoli O; Chemistry Department, Pontifícia Universidade Católica do Rio de Janeiro, 22451-900, Rio de Janeiro, RJ, Brazil., Romani EC; Physics Department, Pontifícia Universidade Católica do Rio de Janeiro, 22451-900 Rio de Janeiro, RJ, Brazil., Aucélio RQ; Chemistry Department, Pontifícia Universidade Católica do Rio de Janeiro, 22451-900, Rio de Janeiro, RJ, Brazil. Electronic address: aucelior@puc-rio.br., da Silva AR; Centro Federal de Educação Tecnológica Celso Suckow da Fonseca-CEFET/RJ, 27600-000 Valença, RJ, Brazil.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2017 Feb 05; Vol. 172, pp. 126-134. Date of Electronic Publication: 2016 Apr 11.
DOI: 10.1016/j.saa.2016.04.023
Abstrakt: Gentamicin is an antibiotic indicated to treat mastitis in dairy cattle and for the treatment of bacterial resistance in the context of hospital infections. The effect caused by gentamicin on the optical properties of gold nanoparticles aqueous dispersions were used to develop quantitative methods to determine this antibiotic. Two different aqueous dispersions, one containing spherical Au nanoparticles (AuNPs) and the other containing Au nanorods (AuNRs), had their conditions adjusted to enable a stable and sensitive response towards gentamicin. The use of AuNPs, with measurement at 681nm of the rising coupling plasmon band, enabled a limit of detection (LOD) of 0.4ngmL -1 (0.02ng absolute LOD), ten times lower than the one achieved by measuring the decreasing of the longitudinal surface plasmon resonance band (at 662nm). The linear analytical response of AuNPs measured at 681nm did not require rationing of signal values to correct for linearity. Stability of the analytical response resulted in intermediary precision below 2%. No significant interference was imposed by excipients traditionally present in injectable solutions for veterinary use. Percent recoveries obtained in such formulations were between 94.5 and 98.2% regardless the existence of any difference in the proportion of the compounds known as gentamicin (C 1 , C 1a and C 2 ) in standard and in the samples. The method requires no derivatization with toxic reagents as usually is required in other spectroscopic approaches.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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