Evaluation of Antitumor Activity of Long-Circulating and pH-Sensitive Liposomes Containing Ursolic Acid in Animal Models of Breast Tumor and Gliosarcoma.

Autor:	Rocha TG; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Lopes SC; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Cassali GD; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Ferreira Ê; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Veloso ES; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Leite EA; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Braga FC; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Ferreira LA; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Balvay D; INSERM U970 , Université Paris Descartes, France., Garofalakis A; INSERM U970 , Université Paris Descartes, France., Oliveira MC; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil itabra2001@yahoo.com.br., Tavitian B; INSERM U970 , Université Paris Descartes, France.
Jazyk:	angličtina
Zdroj:	Integrative cancer therapies [Integr Cancer Ther] 2016 Dec; Vol. 15 (4), pp. 512-524. Date of Electronic Publication: 2016 Apr 29.
DOI:	10.1177/1534735416628273
Abstrakt:	Background Ursolic acid (UA) is a triterpene found in different plant species, possessing antitumor activity, which may be a result of its antiangiogenic effect. However, UA has low water solubility, which limits its use because the bioavailability is impaired. To overcome this inconvenience, we developed long-circulating and pH-sensitive liposomes containing ursolic acid (SpHL-UA). We investigated the antiangiogenic effect of free UA and SpHL-UA in murine brain cancer and human breast tumor models by means of determination of the relative tumor volume, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), and histopathological analysis. Methods The animals were treated with dimethyl sulfoxide in 0.9% (w/v) NaCl, free UA, long-circulating and pH-sensitive liposomes without drug (SpHL), or SpHL-UA. The animals were submitted to each treatment by intraperitoneal injection for 5 days. The dose of free UA or SpHL-UA was equal to 23 mg/kg. Results Tumor growth inhibition was not observed in human breast tumor-bearing animals. For murine gliosarcoma-bearing animals, a slight tumor growth inhibition was observed in the groups treated with free UA or SpHL-UA (9% and 15%, respectively). No significant change in any of the parameters evaluated by DCE-MRI for both experimental models could be observed. Nevertheless, the evaluation of the mean values of magnetic resonance parameters of human breast tumor-bearing animals showed evidence of a possible antiangiogenic effect induced by SpHL-UA. Histopathological analysis did not present significant change for any treatment. Conclusion SpHL-UA did not show antiangiogenic activity in a gliosarcoma model and seemed to induce an antiangiogenic effect in the human breast tumor model. (© The Author(s) 2016.)
Databáze:	MEDLINE
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