| Autor: |
Shakya P; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Sharma AK; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Kumar N; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Vellachi R; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Mathew DD; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Dubey P; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Singh K; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Shrivastava S; Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Shrivastava S; Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Maiti SK; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Hasan A; Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India., Singh KP; Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India. |
| Abstrakt: |
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats. |
