Use of lipidomics to investigate sebum dysfunction in juvenile acne.

Autor:	Camera E; Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy camera@ifo.it., Ludovici M; Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy., Tortorella S; Laboratory of Cheminformatics and Molecular Modeling, Department of Chemistry, Biology, and Biotechnology, University of Perugia, Perugia, Italy., Sinagra JL; Acne Unit, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy., Capitanio B; Acne Unit, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy., Goracci L; Laboratory of Cheminformatics and Molecular Modeling, Department of Chemistry, Biology, and Biotechnology, University of Perugia, Perugia, Italy., Picardo M; Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy.
Jazyk:	angličtina
Zdroj:	Journal of lipid research [J Lipid Res] 2016 Jun; Vol. 57 (6), pp. 1051-8. Date of Electronic Publication: 2016 Apr 27.
DOI:	10.1194/jlr.M067942
Abstrakt:	Acne is a multifactorial skin disorder frequently observed during adolescence with different grades of severity. Multiple factors centering on sebum secretion are implicated in acne pathogenesis. Despite the recognized role of sebum, its compositional complexity and limited analytical approaches have hampered investigation of alterations specifically associated with acne. To examine the profiles of lipid distribution in acne sebum, 61 adolescents (29 males and 32 females) were enrolled in this study. Seventeen subjects presented no apparent clinical signs of acne. The 44 affected individuals were clinically classified as mild (13 individuals), moderate (19 individuals), and severe (12 individuals) acne. Sebum was sampled from the forehead with Sebutape(TM) adhesive patches. Profiles of neutral lipids were acquired with rapid-resolution reversed-phase/HPLC-TOF/MS in positive ion mode. Univariate and multivariate statistical analyses led to the identification of lipid species with significantly different levels between healthy and acne sebum. The majority of differentiating lipid species were diacylglycerols (DGs), followed by fatty acyls, sterols, and prenols. Overall, the data indicated an association between the clinical grading of acne and sebaceous lipid fingerprints and highlighted DGs as more abundant in sebum from adolescents affected with acne. (Copyright © 2016 by the American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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