Modulator effect of a polysaccharide-rich extract from Caesalpinia ferrea stem barks in rat cutaneous wound healing: Role of TNF-α, IL-1β, NO, TGF-β.

Autor: Pereira Lde P; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil., Mota MR; Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, 60430-160 Fortaleza-CE, Brazil., Brizeno LA; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil., Nogueira FC; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil; Faculdade de Educação, Ciências e Letras do Sertão Central, Universidade Estadual do Ceará, Rua José de Queiroz 2554, 63900-000 Quixadá-CE, Brazil., Ferreira EG; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil., Pereira MG; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil; Faculdade de Educação, Ciências e Letras do Sertão Central, Universidade Estadual do Ceará, Rua José de Queiroz 2554, 63900-000 Quixadá-CE, Brazil., Assreuy AM; Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, 60740-000 Fortaleza-CE, Brazil. Electronic address: anassreuy@gmail.com.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2016 Jul 01; Vol. 187, pp. 213-23. Date of Electronic Publication: 2016 Apr 26.
DOI: 10.1016/j.jep.2016.04.043
Abstrakt: Ethnopharmacological Relevance: In folk medicine stem barks of Caesalpinia ferrea (Caesalpinioideae) are used to treat enterocolitis, rheumatism and wounds and in experimental procedures, its aqueous extracts demonstrated antiulcer, anti-inflammatory, analgesic, and healing effects.
Aim of the Study: The healing mechanism of the polyssacharide-rich extract of C. ferrea stem barks (TPL-Cf) was investigated in a model of excisional cutaneous wound in Wistar rats.
Materials and Methods: Excisional wounds received topical treatment with TPL-Cf (0.025-0.1%) during 21 days. Hypernociception, macroscopical, histological and immunohistochemical parameters were evaluated and analyzed by ANOVA, Bonferroni and Kruskal-Wallis tests, followed by Dunn and Chi-Square tests.
Results: TPL-Cf (0.1%) reduced wound area and hypernociception, and increased wound contraction. TPL-Cf reduced leukocyte infiltration and vascular permeability, and stimulated fibroblasia, angiogenesis, well formed granulation tissue, collagen deposition and epithelial layer formation. TPL-Cf reduced TNF-α expression and the levels of PGE2 (73%-day 5), IL-1 (42%-day 2), MDA (38%-day 5), total protein (53%-day 2; 73%-day 5) and MPO activity (53%-day 2), but increased the expression of i-NOS (days 5 and 7), TGF-β (day 5) and the levels of NO (3.6 fold-day 5).
Conclusion: The polysaccharide-rich extract of C. ferra stem barks accelerates wound healing by the control of the inflammatory phase and attenuates hypernociception via modulation of inflammatory mediators (TNF-α, IL-1β, NO, TGF-β).
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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