Diversity in cell motility reveals the dynamic nature of the formation of zebrafish taste sensory organs.

Autor:	Soulika M; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Kaushik AL; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Mathieu B; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Lourenço R; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Komisarczuk AZ; Developmental Neurobiology and Genomics, Brain and Mind Research Institute, University of Sydney, Camperdown, NSW 2050, Australia., Romano SA; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Jouary A; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Lardennois A; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France., Tissot N; Institut Jacques Monod, CNRS UMR7592, University Paris Diderot, 75013 Paris, France., Okada S; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan., Abe K; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan., Becker TS; Developmental Neurobiology and Genomics, Brain and Mind Research Institute, University of Sydney, Camperdown, NSW 2050, Australia., Kapsimali M; IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France marika.kapsimali@ens.fr.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2016 Jun 01; Vol. 143 (11), pp. 2012-24. Date of Electronic Publication: 2016 Apr 27.
DOI:	10.1242/dev.134817
Abstrakt:	Taste buds are sensory organs in jawed vertebrates, composed of distinct cell types that detect and transduce specific taste qualities. Taste bud cells differentiate from oropharyngeal epithelial progenitors, which are localized mainly in proximity to the forming organs. Despite recent progress in elucidating the molecular interactions required for taste bud cell development and function, the cell behavior underlying the organ assembly is poorly defined. Here, we used time-lapse imaging to observe the formation of taste buds in live zebrafish larvae. We found that tg(fgf8a.dr17)-expressing cells form taste buds and get rearranged within the forming organs. In addition, differentiating cells move from the epithelium to the forming organs and can be displaced between developing organs. During organ formation, tg(fgf8a.dr17) and type II taste bud cells are displaced in random, directed or confined mode relative to the taste bud they join or by which they are maintained. Finally, ascl1a activity in the 5-HT/type III cell is required to direct and maintain tg(fgf8a.dr17)-expressing cells into the taste bud. We propose that diversity in displacement modes of differentiating cells acts as a key mechanism for the highly dynamic process of taste bud assembly. (© 2016. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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