Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation.

Autor: Marcondes Sari MH; Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil., Souza AC; Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Rosa SG; Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil., Chagas PM; Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil., da Luz SC; Departamento de Patologia, Universidade Federal de Santa Maria, Zip Code 97105-900 RS, Brazil., Rodrigues OE; Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil., Nogueira CW; Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil. Electronic address: criswn@ufsm.br.
Jazyk: angličtina
Zdroj: European journal of pharmacology [Eur J Pharmacol] 2016 Jun 15; Vol. 781, pp. 25-35. Date of Electronic Publication: 2016 Apr 18.
DOI: 10.1016/j.ejphar.2016.03.051
Abstrakt: This study investigated the potential p-chloro-selenosteroid (PCS) anti-inflammatory effect in different animal models of acute inflammation. In order to determine a time- and a dose-curve response of action, female adult Swiss mice (25-35g) were divided in different groups and pretreated by the intragastric route (i.g.) with PCS (5-10mg/kg) and after the specific times (5, 30 and 60min) the ear inflammation was induced with croton oil (2.5%, 20μl). The ear edema, myeloperoxidase (MPO) activity and histological analyses were performed. In a second experiment, the pleurisy model was used to determine the PCS protective effect (10mg/kg, i.g., 30min before induction) in the inflammatory and oxidative alterations induced by an intrapleural injection of a 1% carrageenan solution (0.1ml) in exudate and lung samples. Dexamethasone (1mg/kg, i.g.) was used as positive control for both models. Statistical analysis was performed through a One-Way ANOVA test followed by the Newman-Keuls' test. Pretreatment of 30min with PCS, only at a dose of 10mg/kg, decreased ear edema and the MPO activity as well as the histological alterations induced by croton oil. In the pleurisy model, PCS (10mg/kg, i.g.; 30min) reduced the leukocyte counts, histological alterations, MPO and adenosine deaminase activities, oxidative damage and the non-enzymatic antioxidant defense imbalance. PCS had a similar anti-inflammatory profile to dexamethasone; however, it showed a better antioxidant effect. PCS had anti-inflammatory and antioxidant actions in two well established inflammation models in mice.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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