Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil.

Autor:	Pereira JN; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil. Electronic address: ju-biomed@hotmail.com., Rabelo MA; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil., Lima JL; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil., Neto AM; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil., Lopes AC; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil., Maciel MA; Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Jazyk:	angličtina
Zdroj:	The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2016 May-Jun; Vol. 20 (3), pp. 276-81. Date of Electronic Publication: 2016 Apr 16.
DOI:	10.1016/j.bjid.2016.03.003
Abstrakt:	Introduction: There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective: To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. Methods: The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller-Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. Results: The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. Conclusion: In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches. (Copyright © 2016 Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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