Chemical characteristics of a heteropolysaccharide from Tylopilus ballouii mushroom and its antioxidant and anti-inflammatory activities.

Autor: Lima AT; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Santos MN; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., de Souza LA; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Pinheiro TS; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Paiva AA; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Dore CM; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Costa MS; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Santos ND; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Baseia YG; Laboratory of Mycology, Department of Botany, Zoology and Ecology, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Araújo RM; Institute of Chemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil., Leite EL; Laboratory of Glycobiology, Department of Biochemistry, CB, Federal University of Rio Grande do Norte, Av. Salgado Filho 3000, Bairro L. Nova, CEP 59072-970 Natal, RN, Brazil. Electronic address: eddaleite@cb.ufrn.br.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2016 Jun 25; Vol. 144, pp. 400-9. Date of Electronic Publication: 2016 Feb 26.
DOI: 10.1016/j.carbpol.2016.02.050
Abstrakt: Fucogalactomannan (FGM) is a non-sulphated polysaccharide isolated from the Tylopilus ballouii mushroom. We investigated the chemical characteristics of this FGM using HPLC, chemical methods, and NMR studies ((1)H, (13)C, (1)H/(13)C-HSQC and DEPT-135 spectroscopies) without chemical fragmentation. This polysaccharide consisted primarily of mannose and galactose with variable amounts of fucose and traces of xylose and with MW of 140kDa. Infrared and NMR spectroscopies showed the possible interaction between these polysaccharides and proteins. The antioxidant activity showed for FGM a high inhibition of superoxide and hydroxyl radicals with an IC50 of 1.25 and 1.6mg/mL, respectively. The results of peroxidation tests showed that FGM had an IC50 of 1.72mg/mL. Furthermore, the anti-inflammatory assay showed that FGM reduced edema by 32.8%, 42.0%, and 56% at doses of 30, 50, and 70mg/kg, respectively. Thus, these results suggested a structure and indicated possible anti-inflammatory and antioxidant activities use of these polysaccharides.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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