Evidence that hematopoietic stem cell function is preserved during aging in long-lived S6K1 mutant mice.

Autor:	Selman C; Glasgow Ageing Research Network (GARNER), Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Sinclair A; Glasgow Ageing Research Network (GARNER), Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Pedroni SM; Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College, London, UK., Irvine EE; Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College, London, UK., Michie AM; Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Withers DJ; Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College, London, UK.
Jazyk:	angličtina
Zdroj:	Oncotarget [Oncotarget] 2016 May 24; Vol. 7 (21), pp. 29937-43.
DOI:	10.18632/oncotarget.8729
Abstrakt:	The mechanistic target of rapamycin (mTOR) signalling pathway plays a highly conserved role in aging; mice lacking ribosomal protein S6 kinase 1 (S6K1-/-) have extended lifespan and healthspan relative to wild type (WT) controls. Exactly how reduced mTOR signalling induces such effects is unclear, although preservation of stem cell function may be important. We show, using gene expression analyses, that there was a reduction in expression of cell cycle genes in young (12 week) and aged (80 week) S6K1-/- BM-derived c-Kit+ cells when compared to age-matched WT mice, suggesting that these cells are more quiescent in S6K1-/- mice. In addition, we investigated hematopoietic stem cell (HSC) frequency and function in young and aged S6K1-/-and WT mice. Young, but not aged, S6K1-/-mice had more LSK (lineage-, c-Kit+, Sca-1+) cells (% of bone marrow (BM)), including the most primitive long-term repopulating HSCs (LT-HSC) relative to WT controls. Donor-derived engraftment of LT-HSCs in recipient mice was unaffected by genotype in young mice, but was enhanced in transplants using LT-HSCs derived from aged S6K1-/- mice. Our results are the first to provide evidence that age-associated HSC functional decline is ameliorated in a long-lived mTOR mutant mouse. Competing Interests: The authors declare no conflicts of interest.
Databáze:	MEDLINE
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