Chromosomal-Level Assembly of the Asian Seabass Genome Using Long Sequence Reads and Multi-layered Scaffolding.

Autor: Vij S; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Kuhl H; Max Planck Institute for Molecular Genetics, Berlin, Germany., Kuznetsova IS; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore.; Laboratory of Chromosome Structure and Function, Department of Cytology and Histology, Biological Faculty, Saint Petersburg State University, St. Petersburg, Russia., Komissarov A; Theodosius Dobzhansky Center for Genome Bioinformatics, Saint Petersburg State University, St. Petersburg, Russia., Yurchenko AA; Theodosius Dobzhansky Center for Genome Bioinformatics, Saint Petersburg State University, St. Petersburg, Russia., Van Heusden P; South African MRC Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa., Singh S; Pacific Biosciences, Menlo Park, California, United States of America., Thevasagayam NM; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Prakki SR; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Purushothaman K; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Saju JM; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Jiang J; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Mbandi SK; South African MRC Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa., Jonas M; South African MRC Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa., Hin Yan Tong A; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada., Mwangi S; South African MRC Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa., Lau D; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Ngoh SY; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Liew WC; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Shen X; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore., Hon LS; Pacific Biosciences, Menlo Park, California, United States of America., Drake JP; Pacific Biosciences, Menlo Park, California, United States of America., Boitano M; Pacific Biosciences, Menlo Park, California, United States of America., Hall R; Pacific Biosciences, Menlo Park, California, United States of America., Chin CS; Pacific Biosciences, Menlo Park, California, United States of America., Lachumanan R; Pacific Biosciences, Menlo Park, California, United States of America., Korlach J; Pacific Biosciences, Menlo Park, California, United States of America., Trifonov V; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Kabilov M; Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Tupikin A; Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Green D; Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Moxon S; The Genome Analysis Centre, Norwich, United Kingdom., Garvin T; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York, United States of America., Sedlazeck FJ; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York, United States of America.; Department of Computer Science, Johns Hopkins University, Baltimore, Maryland, United States of America., Vurture GW; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York, United States of America., Gopalapillai G; Nutrition, Genetics & Biotechnology Division, ICAR-Central Institute of Brackishwater Aquaculture, Tamil Nadu, India., Kumar Katneni V; Nutrition, Genetics & Biotechnology Division, ICAR-Central Institute of Brackishwater Aquaculture, Tamil Nadu, India., Noble TH; College of Marine and Environmental Sciences and Center for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia., Scaria V; CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), New Delhi, India., Sivasubbu S; CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), New Delhi, India., Jerry DR; College of Marine and Environmental Sciences and Center for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia., O'Brien SJ; Theodosius Dobzhansky Center for Genome Bioinformatics, Saint Petersburg State University, St. Petersburg, Russia.; Oceanographic Center, Nova Southeastern University Ft. Lauderdale, Ft. Lauderdale, Florida, United States of America., Schatz MC; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York, United States of America.; Department of Computer Science, Johns Hopkins University, Baltimore, Maryland, United States of America., Dalmay T; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Turner SW; Pacific Biosciences, Menlo Park, California, United States of America., Lok S; The Centre for Applied Genomics, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, Ontario, Canada., Christoffels A; South African MRC Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa., Orbán L; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore.; Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary.; Centre for Comparative Genomics, Murdoch University, Murdoch, Australia.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2016 Apr 15; Vol. 12 (4), pp. e1005954. Date of Electronic Publication: 2016 Apr 15 (Print Publication: 2016).
DOI: 10.1371/journal.pgen.1005954
Abstrakt: We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. SNP analyses identified high levels of genetic diversity and confirmed earlier indications of a population stratification comprising three clades with signs of admixture apparent in the South-East Asian population. The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics.
Databáze: MEDLINE