| Autor: |
Calixto Júnior JT; Post Graduation Biotechnological Programme - RENORBIO, Laboratory of Natural Products, State University of Ceará, Itaperi Campus, Fortaleza, Ceará, Brazil., de Morais SM; Post Graduation Biotechnological Programme - RENORBIO, Laboratory of Natural Products, State University of Ceará, Itaperi Campus, Fortaleza, Ceará, Brazil., Gomez CV; Centro para el Desarrollo de la Investigación Científica (CEDIC), Fundación Moisés Bertoni/Laboratorios Díaz Gill, Asunción, Paraguay., Molas CC; Centro para el Desarrollo de la Investigación Científica (CEDIC), Fundación Moisés Bertoni/Laboratorios Díaz Gill, Asunción, Paraguay., Rolon M; Centro para el Desarrollo de la Investigación Científica (CEDIC), Fundación Moisés Bertoni/Laboratorios Díaz Gill, Asunción, Paraguay., Boligon AA; Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul State, Brazil., Athayde ML; Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul State, Brazil., de Morais Oliveira CD; Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará State, Brazil., Tintino SR; Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará State, Brazil., Henrique Douglas MC; Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará State, Brazil. |
| Abstrakt: |
This work describes the antiparasitic and cytotoxic activities of three plant species from the Cerrado biome, Northeastern Brazil. Significant antiparasitic inhibition was observed against Trypanosoma cruzi (63.86%), Leishmania brasiliensis (92.20%) and Leishmania infantum (95.23%) when using ethanol extract from leaves of Guazuma ulmifolia Lam. (Malvaceae), at a concentration of 500 μg/mL. However, low levels of inhibition were observed when assessing leishmanicidal and trypanocidal (Clone CL-B5) activities of crude ethanol extracts from leaves and bast tissue of Luehea paniculata (Malvaceae) and leaves and bark of Prockia crucis (Salicaceae) at a concentration of 500 μg/mL. The extracts revealed the presence of phenolic acids such as gallic acid, chlorogenic acid, caffeic acid and rosmarinic acid, as well as flavonoids such as rutin, luteolin, apigenin and quercetin - the latter detected only in G. ulmifolia. G. ulmifolia extract displayed higher leishmanicidal activity probably due to the presence of quercetin, a potent known leishmanicidal compound. A cytotoxicity test indicated values over 50% at the highest concentration (1000 μg/mL) for all natural products, which were considered cytotoxic. This points out the need for further tests to enable future in vivo trials, including antineoplastic activity on human tumor cells. |
