Circulating Tumor Cell Count Correlates with Colorectal Neoplasm Progression and Is a Prognostic Marker for Distant Metastasis in Non-Metastatic Patients.

Autor:	Tsai WS; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan.; Graduate Institute of Clinical Medical Science, Chang Gung University, Taoyuan, Taiwan., Chen JS; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan.; Graduate Institute of Clinical Medical Science, Chang Gung University, Taoyuan, Taiwan., Shao HJ; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan., Wu JC; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan., Lai JM; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan., Lu SH; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan.; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan., Hung TF; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan., Chiu YC; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan., You JF; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Hsieh PS; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Yeh CY; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Hung HY; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Chiang SF; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Lin GP; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Tang R; Division of Colon and Rectal Surgery, Colorectal Section, Department of Surgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, 5 Fu-Hsing St. Kueishan, Taoyuan, Taiwan., Chang YC; Genomics Research Center, Academia Sinica, 128, Sec. 2, Academic Rd., Nankang, Taipei 115, Taiwan.; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2016 Apr 14; Vol. 6, pp. 24517. Date of Electronic Publication: 2016 Apr 14.
DOI:	10.1038/srep24517
Abstrakt:	Enumeration of circulating tumor cells (CTCs) has been proven as a prognostic marker for metastatic colorectal cancer (m-CRC) patients. However, the currently available techniques for capturing and enumerating CTCs lack of required sensitivity to be applicable as a prognostic marker for non-metastatic patients as CTCs are even more rare. We have developed a microfluidic device utilizing antibody-conjugated non-fouling coating to eliminate nonspecific binding and to promote the multivalent binding of target cells. We then established the correlation of CTC counts and neoplasm progression through applying this platform to capture and enumerate CTCs in 2 mL of peripheral blood from healthy (n = 27), benign (n = 21), non-metastatic (n = 95), and m-CRC (n = 15) patients. The results showed that the CTC counts progressed from 0, 1, 5, to 36. Importantly, after 2-year follow-up on the non-metastatic CRC patients, we found that those who had ≥5 CTCs were 8 times more likely to develop distant metastasis within one year after curable surgery than those who had <5. In conclusion, by employing a sensitive device, CTC counts show good correlation with colorectal neoplasm, thus CTC may be as a simple, independent prognostic marker for the non-metastatic CRC patients who are at high risk of early recurrence.
Databáze:	MEDLINE
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