Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy.

Autor: Rajan KS; Cardiac Hypertrophy Laboratory, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Velmurugan G; Cardiac Hypertrophy Laboratory, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Gopal P; Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Ramprasath T; Center for Molecular and Translational Medicine, Georgia State University, Atlanta, GA, USA., Babu DD; Cardiac Hypertrophy Laboratory, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Krithika S; Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Jenifer YC; Cardiac Hypertrophy Laboratory, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India., Freddy A; Deparment of Zoology, Madras Christian College, Chennai, 600 059, Tamilnadu, India., William G Jn; Department of Clinical & Experimental Medicine, University Linkoping, Linkoping, Sweden., Kalpana K; Department of Plant Pathology, Regional Research Station, Tamilnadu Agricultural University, Krishangiri, Tamilnadu, India., Ramasamy S; Cardiac Hypertrophy Laboratory, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India. Electronic address: subbiahr@nrcbsmku.org.
Jazyk: angličtina
Zdroj: Heart, lung & circulation [Heart Lung Circ] 2016 Oct; Vol. 25 (10), pp. 1013-20. Date of Electronic Publication: 2016 Mar 28.
DOI: 10.1016/j.hlc.2016.02.015
Abstrakt: Background: The discovery of PIWI-interacting RNAs (piRNAs) has fundamentally changed our understanding of post transcriptional regulation of transposons and other genes. Unlike miRNA and siRNA, the piRNAs are the most abundant but least studied RNA species in mammals. Although the expression of PIWI proteins and piRNAs has long been regarded as germline specific, increasing evidences suggest the expression of piRNAs in somatic cells.
Methods: In this study, the small RNA sequencing executed during induction of cardiac hypertrophy in both in vivo and in vitro conditions were annotated for the expression of piRNAs. The expression of piRNAs was validated by qPCR and RNA immunoprecipitation. In addition, the presence of piRNAs in circulation of myocardial infarction patients was studied by qPCR.
Results: We identified an abundant and altered expression of piRNAs during cardiac hypertrophy. The differentially expressed piRNAs was validated by qPCR and RNA immunoprecipitation. The significantly and differentially expressed piRNAs were predicted to target different retrotransposons and mRNAs in the rat genome. The detection of specific piRNA in serum of myocardial infarction patients suggests the potential of piRNA for diagnosis.
Conclusion: Overall this study is the first to provide a whole-genome analysis of the large repertoire of piRNAs in the cardiac system and this would pave a new path to understanding the molecular aetiology of piRNA and retrotransposons in the physiology and pathology of the cardiac system.
(Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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