Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations.

Autor: Ferreira PMP; Laboratory in Experimental Cancerology, Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil; Postgraduate Program in Biotechnology, Federal University of Piauí, Teresina, Brazil. Electronic address: pmpf@ufpi.edu.br., Bezerra DP; Oswaldo Cruz Foundation, Salvador, Brazil., Silva JDN; Postgraduate Program in Biotechnology, Federal University of Piauí, Teresina, Brazil., da Costa MP; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil; Department of Pharmacy, Federal University of Piauí, Teresina, Brazil., Ferreira JRO; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Alencar NMN; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil., Figueiredo IST; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil., Cavalheiro AJ; Chemistry Institute, State University of São Paulo, Araraquara, Brazil., Machado CML; Radioisotopes Research Laboratory, Center for Medical Nuclear, State University of São Paulo, São Paulo, Brazil., Chammas R; Department of Radiology, Faculty of Medicine, State University of São Paulo, São Paulo, Brazil., Alves APNN; Department of Clinical Odontology, Federal University of Ceará, Fortaleza, Brazil., Moraes MO; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil., Pessoa C; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil; Oswaldo Cruz Foundation, Fortaleza, Brazil.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2016 Jun 20; Vol. 186, pp. 270-279. Date of Electronic Publication: 2016 Apr 08.
DOI: 10.1016/j.jep.2016.04.011
Abstrakt: Ethnopharmacological Relevance: Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities.
Aim of the Study: To assess the in vivo and ex vivo antitumor action of a fraction with casearins (FC) and its main component - Casearin X-isolated from C. sylvestris leaves.
Materials and Methods: Firstly, Sarcoma 180 bearing Swiss mice were treated with FC and Cas X for 7 days. Secondly, BALB/c nude animals received hollow fibers with colon carcinoma (HCT-116) or glioblastoma (SF-295) cells and were treated with FC for 4 days. On 5th day, proliferation was determined by MTT assay.
Results: FC 10 and 25mg/kg/day i.p. and 50mg/kg/day oral and Cas X 25mg/kg/day i.p. and 50mg/kg/day oral revealed tumor growth inhibition rates of 35.8, 86.2, 53.7, 90.0 and 65.5% and such tumors demonstrated rare mitoses and coagulation necrosis areas. Similarly, FC reduced multiplying of HCT-116 and SF-295 cells when evaluated by the Hollow Fiber Assay (2.5 and 5mg/kg/day i.p. and 25 and 50mg/kg/day oral), with cell growth inhibition rates ranging from 33.3 to 67.4% (p<0.05). Flow cytometry experiments revealed that FC reduced membrane integrity and induced DNA fragmentation and mitochondrial depolarization (p<0.05).
Conclusions: FC and Cas X were efficient antitumor substances against murine and human cancer cells and caused reversible morphological changes in liver, kidneys and spleens, emphasizing clerodane diterpenes as an emerging class of anticancer molecules.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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