NUP98-PHF23 fusion is recurrent in acute myeloid leukemia and shares gene expression signature of leukemic stem cells.

Autor: Ho H; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Skaist AM; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Pallavajjala A; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Yonescu R; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Batista D; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Wheelan SJ; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Ning Y; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, United States. Electronic address: yning5@jhmi.edu.
Jazyk: angličtina
Zdroj: Leukemia research [Leuk Res] 2016 Jun; Vol. 45, pp. 1-7. Date of Electronic Publication: 2016 Mar 30.
DOI: 10.1016/j.leukres.2016.03.006
Abstrakt: Chromosome translocations involving nucleoporin 98 gene (NUP98) have been identified in a wide array of hematologic malignancies, and the resulting NUP98-associated fusions are known to play a critical role in leukemogensis through dysregulation of gene expression. Although NUP98-associated fusions were initially thought to be rare, application of molecular technologies has revealed that cryptic translocations involving NUP98 are more frequent than previously appreciated. We report an additional case of t(11;17)(p15;p13) resulting in the fusion of NUP98 and plant homeodomain finger 23 (PHF23) in a pediatric patient with acute myeloid leukemia (AML). Using RNA sequencing, we determined in-frame fusion points and also analyzed the gene expression profile of NUP98-PHF23 positive AML. Gene set enrichment analysis (GSEA) demonstrates that NUP98-PHF23 fusion shares gene expression signature of NUP98-HOXA9 fusion, the prototype of the NUP98-associated fusions, as well as the signature of leukemic stem cells. To our knowledge this is the first transcriptome analysis of human samples with NUP98-PHF23 positive AML. Our findings are in support of the gene expression study of NUP98-PHF23 mouse model and validate the usefulness of the mouse model in developing therapeutic strategies for the treatment of subsets of AML.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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