Molecular characterization and phylogeny of Shiga toxin-producing Escherichia coli isolates obtained from two Dutch regions using whole genome sequencing.

Autor:	Ferdous M; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Friedrich AW; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Grundmann H; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., de Boer RF; Department of Medical Microbiology, Certe Laboratory for Infectious Diseases, Groningen, The Netherlands., Croughs PD; Star-MDC, Rotterdam, The Netherlands., Islam MA; Department of Psychiatry, Rob Giel Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Kluytmans-van den Bergh MF; Julius Center for Health Sciences and Primary Care, UMC Utrecht, Utrecht, The Netherlands; Amphia Academy Infectious Disease Foundation, Amphia Hospital, Breda, The Netherlands., Kooistra-Smid AM; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Department of Medical Microbiology, Certe Laboratory for Infectious Diseases, Groningen, The Netherlands., Rossen JW; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: j.rossen@umcg.nl.
Jazyk:	angličtina
Zdroj:	Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2016 Jul; Vol. 22 (7), pp. 642.e1-9. Date of Electronic Publication: 2016 Apr 04.
DOI:	10.1016/j.cmi.2016.03.028
Abstrakt:	Shiga toxin-producing Escherichia coli (STEC) is one of the major causes of human gastrointestinal disease and has been implicated in sporadic cases and outbreaks of diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome worldwide. In this study, we determined the molecular characteristics and phylogenetic relationship of STEC isolates, and their genetic diversity was compared to that of other E. coli populations. Whole genome sequencing was performed on 132 clinical STEC isolates obtained from the faeces of 129 Dutch patients with gastrointestinal complaints. STEC isolates of this study belonged to 44 different sequence types (STs), 42 serogenotypes and 14 stx subtype combinations. Antibiotic resistance genes were more frequently present in stx1-positive isolates compared to stx2 and stx1 + stx2-positive isolates. The iha, mchB, mchC, mchF, subA, ireA, senB, saa and sigA genes were significantly more frequently present in eae-negative than in eae-positive STEC isolates. Presence of virulence genes encoding type III secretion proteins and adhesins was associated with isolates obtained from patients with bloody diarrhoea. Core genome phylogenetic analysis showed that isolates clustered according to their ST or serogenotypes irrespective of stx subtypes. Isolates obtained from patients with bloody diarrhoea were from diverse phylogenetic backgrounds. Some STEC isolates shared common ancestors with non-STEC isolates. Whole genome sequencing is a powerful tool for clinical microbiology, allowing high-resolution molecular typing, population structure analysis and detailed molecular characterization of strains. STEC isolates of a substantial genetic diversity and of distinct phylogenetic groups were observed in this study. (Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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