Association of Human Antibodies to Arabinomannan With Enhanced Mycobacterial Opsonophagocytosis and Intracellular Growth Reduction.
Autor: | Chen T; Department of Medicine., Blanc C; Department of Medicine., Eder AZ; Department of Medicine., Prados-Rosales R; Department of Microbiology and Immunology., Souza AC; Department of Microbiology and Immunology., Kim RS; Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx., Glatman-Freedman A; Department of Pediatrics Department of Family and Community Medicine, New York Medical College, Valhalla, New York Infectious Diseases Unit, Israel Center for Disease Control, Israel Ministry of Health, Tel Hashomer., Joe M; Alberta Glycomics Centre Department of Chemistry, University of Alberta, Edmonton, Canada., Bai Y; Alberta Glycomics Centre Department of Chemistry, University of Alberta, Edmonton, Canada., Lowary TL; Alberta Glycomics Centre Department of Chemistry, University of Alberta, Edmonton, Canada., Tanner R; Jenner Institute, University of Oxford, United Kingdom., Brennan MJ; Aeras, Rockville., Fletcher HA; Jenner Institute, University of Oxford, United Kingdom., McShane H; Jenner Institute, University of Oxford, United Kingdom., Casadevall A; Department of Medicine Department of Microbiology and Immunology Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland., Achkar JM; Department of Medicine Department of Microbiology and Immunology. |
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Jazyk: | angličtina |
Zdroj: | The Journal of infectious diseases [J Infect Dis] 2016 Jul 15; Vol. 214 (2), pp. 300-10. Date of Electronic Publication: 2016 Apr 07. |
DOI: | 10.1093/infdis/jiw141 |
Abstrakt: | Background: The relevance of antibodies (Abs) in the defense against Mycobacterium tuberculosis infection remains uncertain. We investigated the role of Abs to the mycobacterial capsular polysaccharide arabinomannan (AM) and its oligosaccharide (OS) fragments in humans. Methods: Sera obtained from 29 healthy adults before and after primary or secondary bacillus Calmette-Guerin (BCG) vaccination were assessed for Ab responses to AM via enzyme-linked immunosorbent assays, and to AM OS epitopes via novel glycan microarrays. Effects of prevaccination and postvaccination sera on BCG phagocytosis and intracellular survival were assessed in human macrophages. Results: Immunoglobulin G (IgG) responses to AM increased significantly 4-8 weeks after vaccination (P < .01), and sera were able to opsonize BCG and M. tuberculosis grown in both the absence and the presence of detergent. Phagocytosis and intracellular growth inhibition were significantly enhanced when BCG was opsonized with postvaccination sera (P < .01), and these enhancements correlated significantly with IgG titers to AM (P < .05), particularly with reactivity to 3 AM OS epitopes (P < .05). Furthermore, increased phagolysosomal fusion was observed with postvaccination sera. Conclusions: Our results provide further evidence for a role of Ab-mediated immunity to tuberculosis and suggest that IgG to AM, especially to some of its OS epitopes, could contribute to the defense against mycobacterial infection in humans. (© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.) |
Databáze: | MEDLINE |
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