| Autor: |
Cho YM; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Kim TM; Department of Medical Informatics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Hun Kim D; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Hee Kim D; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Jeong SW; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Kwon OJ; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. |
| Abstrakt: |
Wnt10b, an endogenous inhibitor of adipogenesis, maintains preadipocytes in an undifferentiated state by suppressing adipogenic transcription factors. We have previously demonstrated that Wnt10b transcription during adipogenesis is negatively regulated by X-box-binding protein 1 (XBP1), an important transcription factor of the unfolded protein response. In this report, we demonstrate that XBP1s can directly induce the transcription of microRNA-148a, which in turn mediates the silencing of Wnt10b mRNA during adipogenic differentiation of 3T3-L1 cells. Stability of Wnt10b mRNA was found to be significantly increased by knockdown of XBP1s. Using computational algorithms, a set of microRNAs was predicted to bind Wnt10b mRNA, of which microRNA-148a was selected as a potential target for XBP1s. Our results revealed that microRNA-148a could bind to the 3'UTR of Wnt10b mRNA. Its ectopic expression significantly suppressed both Wnt10b expression and β-catenin activity. When we altered the expression of XBP1 in 3T3-L1 cells, microRNA-148a levels changed accordingly. A potential XBP1 response element was found in the promoter region of microRNA-148a, and XBP1s directly bound to this response element as shown by point mutation analysis and chromatin immunoprecipitation assay. In addition, a microRNA-148a mimic significantly restored adipogenic potential in XBP1-deficient 3T3-L1 cells. These findings provide the first evidence that XBP1s can regulate Wnt10b by a post-transcriptional mechanism through directly inducing microRNA-148a. |
