Near-infrared fluorescence sentinel lymph node detection in gastric cancer: A pilot study.

Autor:	Tummers QR; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Boogerd LS; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., de Steur WO; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Verbeek FP; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Boonstra MC; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Handgraaf HJ; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Frangioni JV; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., van de Velde CJ; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Hartgrink HH; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Vahrmeijer AL; Quirijn RJG Tummers, Leonora SF Boogerd, Wobbe O de Steur, Floris PR Verbeek, Martin C Boonstra, Henricus JM Handgraaf, Cornelis JH van de Velde, Henk H Hartgrink, Alexander L Vahrmeijer, Department of Surgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2016 Apr 07; Vol. 22 (13), pp. 3644-51.
DOI:	10.3748/wjg.v22.i13.3644
Abstrakt:	Aim: To investigate feasibility and accuracy of near-infrared fluorescence imaging using indocyanine green: nanocolloid for sentinel lymph node (SLN) detection in gastric cancer. Methods: A prospective, single-institution, phase I feasibility trial was conducted. Patients suffering from gastric cancer and planned for gastrectomy were included. During surgery, a subserosal injection of 1.6 mL ICG:Nanocoll was administered around the tumor. NIR fluorescence imaging of the abdominal cavity was performed using the Mini-FLARE™ NIR fluorescence imaging system. Lymphatic pathways and SLNs were visualized. Of every detected SLN, the corresponding lymph node station, signal-to-background ratio and histopathological diagnosis was determined. Patients underwent standard-of-care gastrectomy. Detected SLNs outside the standard dissection planes were also resected and evaluated. Results: Twenty-six patients were enrolled. Four patients were excluded because distant metastases were found during surgery or due to technical failure of the injection. In 21 of the remaining 22 patients, at least 1 SLN was detected by NIR Fluorescence imaging (mean 3.1 SLNs; range 1-6). In 8 of the 21 patients, tumor-positive LNs were found. Overall accuracy of the technique was 90% (70%-99%; 95%CI), which decreased by higher pT-stage (100%, 100%, 100%, 90%, 0% for respectively Tx, T1, T2, T3, T4 tumors). All NIR-negative SLNs were completely effaced by tumor. Mean fluorescence signal-to-background ratio of SLNs was 4.4 (range 1.4-19.8). In 8 of the 21 patients, SLNs outside the standard resection plane were identified, that contained malignant cells in 2 patients. Conclusion: This study shows successful use of ICG:Nanocoll as lymphatic tracer for SLN detection in gastric cancer. Moreover, tumor-containing LNs outside the standard dissection planes were identified.
Databáze:	MEDLINE
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