A method for culturing Gram-negative skin microbiota.
| Autor: | Myles IA; Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. mylesi@niaid.nih.gov., Reckhow JD; Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Williams KW; Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Sastalla I; Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Frank KM; Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, MD, USA., Datta SK; Bacterial Pathogenesis Unit, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. |
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| Jazyk: | angličtina |
| Zdroj: | BMC microbiology [BMC Microbiol] 2016 Apr 06; Vol. 16, pp. 60. Date of Electronic Publication: 2016 Apr 06. |
| DOI: | 10.1186/s12866-016-0684-9 |
| Abstrakt: | Background: Commensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described. Results: Through the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures. Conclusions: Our protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease. |
| Databáze: | MEDLINE |
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