Proteome Modification in Tomato Plants upon Long-Term Aluminum Treatment.

Autor: Zhou S; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Okekeogbu I; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Sangireddy S; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Ye Z; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Li H; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Bhatti S; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., Hui D; Department of Agricultural and Environmental Sciences, College of Agriculture, Human and Natural Sciences, Tennessee State University , 3500 John A Merritt Blvd, Nashville, Tennessee 37209, United States., McDonald DW; Phenotype Screening Corporation , 4028 Papermill Road, Knoxville, Tennessee 37909, United States., Yang Y; RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States., Giri S; RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States., Howe KJ; RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States., Fish T; RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States., Thannhauser TW; RW Holley Center for Agriculture and Health, Plant, Soil and Nutrition Research Unit, USDA-ARS , Tower Rd, Ithaca, New York 14853, United States.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2016 May 06; Vol. 15 (5), pp. 1670-84. Date of Electronic Publication: 2016 Apr 18.
DOI: 10.1021/acs.jproteome.6b00128
Abstrakt: This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro-Tom") after long-term exposure to the stress factor. Plants were treated in Magnavaca's solution (pH 4.5) supplemented with 7.5 μM Al(3+) ion activity over a 4 month period beginning at the emergence of flower buds and ending when the lower mature leaves started to turn yellow. Proteomes were identified using a 8-plex isobaric tags for relative and absolute quantification (iTRAQ) labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation and final generation of tandem mass spectrometry (MS/MS) spectra of tryptic peptides on an LTQ-Orbitrap Elite mass spectrometer. Principal component analysis revealed that the Al-treatment had induced systemic alterations in the proteomes from roots and leaves but not seed tissues. The significantly changed root proteins were shown to have putative functions in Al(3+) ion uptake and transportation, root development, and a multitude of other cellular processes. Changes in the leaf proteome indicate that the light reaction centers of photosynthetic machinery are the primary targets of Al-induced stress. Embryo and seed-coat tissues derived from Al-treated plants were enriched with stress proteins. The biological processes involving these Al-induced proteins concur with the physiological and morphological changes, such as the disturbance of mineral homeostasis (higher contents of Al, P, and Fe and reduced contents of S, Zn, and Mn in Al-treated compared to nontreated plants) in roots and smaller sizes of roots and the whole plants. More importantly, the identified significant proteins might represent a molecular mechanism for plants to develop toward establishing the Al tolerance and adaptation mechanism over a long period of stress treatment.
Databáze: MEDLINE
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