The anti-inflammatory and pro-resolution effects of aspirin-triggered RvD1 (AT-RvD1) on peripheral blood mononuclear cells from patients with severe asthma.

Autor: Zambalde ÉP; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: erikazambaldi@gmail.com., Teixeira MM; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: maxellemartins@hotmail.com., Favarin DC; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: danielyfavarin@hotmail.com., de Oliveira JR; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: jhony_sacra@hotmail.com., Magalhães ML; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: mahmagalhaes@gmail.com., Cunha MM; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: maiaramcunha@yahoo.com.br., Silva WC Junior; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: wcsjr@terra.com.br., Okuma CH; Inflammation and Pain Laboratory, Department of Pharmacology, School of Medicine of Ribeirao Preto, University of São Paulo, Brazil. Electronic address: cindhana@hotmail.com., Rodrigues V Junior; Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: vrodrigues@mednet.com.br., Levy BD; Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, United States. Electronic address: blevy@partners.org., Rogerio AP; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil. Electronic address: alexprogerio@biomedicina.uftm.edu.br.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2016 Jun; Vol. 35, pp. 142-148. Date of Electronic Publication: 2016 Apr 16.
DOI: 10.1016/j.intimp.2016.03.014
Abstrakt: Asthma is an inflammatory disease that is characterized by a predominance of eosinophils and/or neutrophils in the airways. In the resolution of inflammation, lipid mediators such as resolvin D1 (RvD1) and its epimer aspirin-triggered RvD1 (AT-RvD1) are produced and demonstrate anti-inflammatory and pro-resolution effects. In experimental models such as airway allergic inflammation induced by ovalbumin in mice, RvD1 and AT-RvD1 alleviate some of the most important phenotypes of asthma. Here, we demonstrated the effects of AT-RvD1 on peripheral blood mononuclear cells (PBMCs) from healthy individuals and patients with severe asthma stimulated with lipopolysaccharide (LPS) or Dermatophagoides pteronyssinus (DM). AT-RvD1 (100nM) reduced the concentration of TNF-α in PBMCs from healthy individuals and patients with severe asthma stimulated with LPS or DM. In addition, AT-RvD1 lowered the production of IL-10 only in PBMCs from patients with severe asthma stimulated with LPS. These effects were associated in part with decreasing NF-κB activation. Moreover, AT-RvD1 significantly increased phagocytosis of apoptotic neutrophils by monocytes from patients with severe asthma. In conclusion, AT-RvD1 demonstrated both anti-inflammatory and pro-resolution effects in PBMCs from patients with severe asthma and could become in the future an alternative treatment for asthma.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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