| Autor: |
Erukainure OL; a Food Technology Division, Federal Institute of Industrial Research , Oshodi , Lagos , Nigeria.; b Medicinal Chemistry Unit, Department of Chemistry, Adamawa State University , Mubi , Nigeria.; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan., Zaruwa MZ; b Medicinal Chemistry Unit, Department of Chemistry, Adamawa State University , Mubi , Nigeria., Choudhary MI; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan., Naqvi SA; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan., Ashraf N; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan., Hafizur RM; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan., Muhammad A; c Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi , Karachi , Pakistan.; d Department of Biochemistry , Ahmadu Bello University , Zaria , Kaduna State , Nigeria., Ebuehi OA; e Department of Biochemistry , University of Lagos , Lagos , Nigeria., Elemo GN; a Food Technology Division, Federal Institute of Industrial Research , Oshodi , Lagos , Nigeria. |
| Abstrakt: |
The antiproliferative effect of the fatty acid components of Clerodendrum volubile leaves as well as its antioxidant effect on MCF-7 and MDA-MB-231 human breast cancer cell lines were investigated. Fatty acids extracted from C. volubile leaf oil were subjected to gas chromatography mass spectrometry (GCMS) analysis. The cells were cultured and treated with the fatty acids for 48 h, after which the antiproliferation effect was ascertained via MTT assay and cell viability analysis using BD fluorescence activated cells sorting (FACS) Calibur. Cell cycle was analyzed by flow cytometry on FACS Calibur. Western blotting was used in determining expression of proteins in the cell lines. The treated cell lines were assessed for reduced glutathione level, catalase, superoxide dismutase, and lipid peroxidation. The fatty acids significantly inhibited cell proliferation, arrested G0/G1 phase, downregulated the expression of MMP-9, and attenuated oxidative stress in of MCF-7 cell lines but had little or no effect on MDA-MB-231 cell lines. These results indicate the therapeutic potential of the fatty acids components of the leaves of C. volubile on human breast cancer, which may be explored further in drug development. |
